‘If you can't beat them, confuse them’: Scientist baffles entitled colleague by showing kindness, leaves him confused in the dust to work a “desirable” PhD position

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  • When I was working in a lab as an unpaid intern while I was waiting for the start of my PhD programme in a different town there was a group leader from a neighbouring group who was a complete j.
  • He kinda reminded me of Odo from DS9 so let's call him that. My boss and him were good enough friends and she needed me to borrow some DNA called a plasmid, which basically is a little circle of DNA that tricks a bacteria into doing two things - making a protein and being resistant to antibiotic.
  • Bacteria are lazy little critters and if you try giving them a plasmid that expresses a protein they don't like then they will do anything to stop having to make it - like grabbing the antibiotic resistance gene, stuffing it in their own DNA and ditching the rest.
  • If you are sensible you always use a strain of bacteria that cannot do this stunt or you won't achieve anything and when you try to purify your precious plasmid from be bacteria you get nothing but water.
  • This is important. I needed to borrow just a few microlitres of a plasmid from him that I knew for a fact bacteria would hate to have.
  • j He acted like a total and gave me a third of what I needed. When I tried to use it to make bacteria resistant to antibiotic (and prove they were expressing the offensive protein) I got nothing.
  • He acted like I was a useless idiot. He gave me some of his stock of bacteria that were already resistant.
  • I grew them up, tried to purity the plasmid and got back water, just water. He didn't believe me again.
  • He demanded I provided proof of the fact that these bacteria had no plasmid. After days and days I presented my evidence and grudgingly he gave me a straight DNA sample from his private stash.
  • This worked an absolute treat, the cells were resistant to antibiotic and everything was as it should be.
  • It was at that point it became clear to me that this big clever group leader had used the wrong strain of bacteria.
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  • Those little critters had ditched the nasty bit of his plasmid and kept the antibiotic resistance all to themselves.
  • This was a rookie mistake and as a rookie I had spotted it. I used the right strain, grew up a whole bunch of the bacteria and made several preps of top quality DNA.
  • I could have just let it lie and let him figure out his own problems. However, instead I decided to do what he least expected.
  • I breezed into his lab, casually dropped off a tube of 50 microlitres of freshly prepped DNA and plate with freshly grown correct bacteria - which would let him prepare a limitless supply.
  • I didn't hang around for him to say anything and breezed on out. A few days later his wife, who worked for him and she was also a piece of work, randomly congratulated me for my upcoming PhD position.
  • This may seem inconsequential but it actually proved my plot had worked. I found out the following bit from talking to others.
  • In the words of Monk - here's what happened... Shortly after I had dropped off the enchanting gift in Odo's lab he had gone over to my boss and expressed total confusion.
  • He could not figure out why I was being nice to him and his first conclusion was that surely I wanted something, perhaps I had been fishing for a job.
  • My boss informed him that I would have had no motive to do that as I already had a very desirable PhD position starting in a few months.
  • This just deepened his bewilderment. I wish I could have seen the look on his face when he realised I had been nice to him for exactly no valid reason.

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